biotinylated anti il 8 ab  (R&D Systems)

Journal:

Article Title: Thrombin Regulates Chemokine Induction during Human Retinal Pigment Epithelial Cell/Monocyte Interaction

doi:

Figure Lengend Snippet: Modulation of IL-8 (A), MCP-1 (B), and IL-6 (C) secretion by IFN-γ. HRPE cells (HRPE), monocytes (Mo), and HRPE cell/monocyte co-cultures (HRPE + Mo) were incubated in the medium containing IFN-γ (1000 U/ml), either alone or in combination with thrombin for 24 hours. Supernatants were collected and IL-8, MCP-1, and IL-6 protein levels in supernatants were measured by ELISA (n = 3).

Article Snippet: Recombinant human (rh) IL-8, rhMCP-1, rhIL-6, rhIL-1β, rhTNF-α, rhIFN-γ, monoclonal anti-IL-8 antibody (Ab), monoclonal anti-MCP-1 Ab, monoclonal anti-IL-6 Ab, monoclonal anti-IL-1β Ab, monoclonal anti-TNF-α Ab, biotinylated anti-IL-8 Ab, biotinylated anti-MCP-1 Ab, biotinylated anti-IL-6 Ab, and biotinylated anti-TNF-α Ab were purchased from R & D Systems (Minneapolis, MN).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Thrombin Regulates Chemokine Induction during Human Retinal Pigment Epithelial Cell/Monocyte Interaction

doi:

Figure Lengend Snippet: Thrombin induction of HRPE IL-8 and MCP-1 mRNA. A: HRPE cells were incubated with thrombin (10 U/ml) for different times, total RNA was extracted, and semiquantitative RT-PCR was performed. These representative data are from one of three independent experiments. B: Results are expressed as a ratio of each PCR product/β-actin band density. Values represent means ± SEM (n = 3).

Article Snippet: Recombinant human (rh) IL-8, rhMCP-1, rhIL-6, rhIL-1β, rhTNF-α, rhIFN-γ, monoclonal anti-IL-8 antibody (Ab), monoclonal anti-MCP-1 Ab, monoclonal anti-IL-6 Ab, monoclonal anti-IL-1β Ab, monoclonal anti-TNF-α Ab, biotinylated anti-IL-8 Ab, biotinylated anti-MCP-1 Ab, biotinylated anti-IL-6 Ab, and biotinylated anti-TNF-α Ab were purchased from R & D Systems (Minneapolis, MN).

Techniques: Incubation, Reverse Transcription Polymerase Chain Reaction

Journal:

Article Title: Thrombin Regulates Chemokine Induction during Human Retinal Pigment Epithelial Cell/Monocyte Interaction

doi:

Figure Lengend Snippet: Thrombin modulated cell-associated IL-8 (A) and MCP-1 (B). HRPE cells (HRPE), monocytes (Mo), and HRPE cell/monocyte co-cultures were incubated with thrombin (10 U/ml) for 4 hours. HRPE cells and monocytes in the co-cultures were separated, and cells were lysed, as described in Materials and Methods. IL-8 and MCP-1 levels were measured by ELISA. c versus d, P < 0.05; a versus b, a versus e, e versus g, e versus f, f versus h, g versus h, i versus j, i versus m, m versus o, m versus n, and n versus p; P < 0.01 (n = 3).

Article Snippet: Recombinant human (rh) IL-8, rhMCP-1, rhIL-6, rhIL-1β, rhTNF-α, rhIFN-γ, monoclonal anti-IL-8 antibody (Ab), monoclonal anti-MCP-1 Ab, monoclonal anti-IL-6 Ab, monoclonal anti-IL-1β Ab, monoclonal anti-TNF-α Ab, biotinylated anti-IL-8 Ab, biotinylated anti-MCP-1 Ab, biotinylated anti-IL-6 Ab, and biotinylated anti-TNF-α Ab were purchased from R & D Systems (Minneapolis, MN).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Thrombin Regulates Chemokine Induction during Human Retinal Pigment Epithelial Cell/Monocyte Interaction

doi:

Figure Lengend Snippet: Cell-cell contact enhanced thrombin induction of IL-8 (A), MCP-1 (B), and IL-6 (C) secretion by co-cultures. HRPE cells co-incubated with monocytes in the same cultures, but separated by porous polycarbonate filters (HRPE/Mo), and HRPE cells overlayed with monocytes directly (HRPE + Mo) were incubated with thrombin (10 U/ml). After 24 hours, supernatants were collected and IL-8, MCP-1, and IL-6 protein levels in supernatants were measured by ELISA. a versus c, b versus d, e versus g, f versus h, i versus k, and j versus l; P < 0.01 (n = 3).

Article Snippet: Recombinant human (rh) IL-8, rhMCP-1, rhIL-6, rhIL-1β, rhTNF-α, rhIFN-γ, monoclonal anti-IL-8 antibody (Ab), monoclonal anti-MCP-1 Ab, monoclonal anti-IL-6 Ab, monoclonal anti-IL-1β Ab, monoclonal anti-TNF-α Ab, biotinylated anti-IL-8 Ab, biotinylated anti-MCP-1 Ab, biotinylated anti-IL-6 Ab, and biotinylated anti-TNF-α Ab were purchased from R & D Systems (Minneapolis, MN).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Thrombin Regulates Chemokine Induction during Human Retinal Pigment Epithelial Cell/Monocyte Interaction

doi:

Figure Lengend Snippet: Anti-TNF-α Ab, but not anti-IL-1β Ab, inhibited thrombin-induced IL-8 (A) and MCP-1 (B) secretion by HRPE cell/monocyte co-cultures. HRPE cell/monocyte co-cultures (HRPE + Mo) were incubated with anti-TNF-α Ab or anti-IL-1β Ab in the presence or absence of thrombin (10 U/ml) for 24 hours. Supernatants were collected and IL-8 and MCP-1 protein levels in supernatants were measured by ELISA (n = 3).

Article Snippet: Recombinant human (rh) IL-8, rhMCP-1, rhIL-6, rhIL-1β, rhTNF-α, rhIFN-γ, monoclonal anti-IL-8 antibody (Ab), monoclonal anti-MCP-1 Ab, monoclonal anti-IL-6 Ab, monoclonal anti-IL-1β Ab, monoclonal anti-TNF-α Ab, biotinylated anti-IL-8 Ab, biotinylated anti-MCP-1 Ab, biotinylated anti-IL-6 Ab, and biotinylated anti-TNF-α Ab were purchased from R & D Systems (Minneapolis, MN).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Thrombin Regulates Chemokine Induction during Human Retinal Pigment Epithelial Cell/Monocyte Interaction

doi:

Figure Lengend Snippet: Effect of thrombin on rhIL-1β-induced (A and B) and rhTNF-α-induced (C and D) IL-8 (A and C), MCP-1 (B and D), and IL-6 (E) secretion. Thrombin had synergistic effects on rhIL-1β- and rhTNF-α-induced IL-8, MCP-1, and IL-6 secretion. HRPE cells (HRPE), monocytes (Mo), and HRPE cell/monocyte co-cultures (HRPE + Mo) were incubated in the medium containing rhIL-1β (0.2 ng/ml) or rhTNF-α (2 ng/ml), either alone or in combination with thrombin for 24 hours. Supernatants were collected and IL-8, MCP-1, and IL-6 protein levels in supernatants were measured by ELISA. *, P < 0.05; **, P < 0.01, compared with chemokine secretion from cells with rhIL-1β or rhTNF-α alone (n = 3).

Article Snippet: Recombinant human (rh) IL-8, rhMCP-1, rhIL-6, rhIL-1β, rhTNF-α, rhIFN-γ, monoclonal anti-IL-8 antibody (Ab), monoclonal anti-MCP-1 Ab, monoclonal anti-IL-6 Ab, monoclonal anti-IL-1β Ab, monoclonal anti-TNF-α Ab, biotinylated anti-IL-8 Ab, biotinylated anti-MCP-1 Ab, biotinylated anti-IL-6 Ab, and biotinylated anti-TNF-α Ab were purchased from R & D Systems (Minneapolis, MN).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay